Activated platelets may adhere to leukocytes and form circulating mixed aggregates, platelet-leucocyte conjugates. The latter are considered a reliable marker of prothrombotic conditions and provide a link between inflammation and thrombosis, two central processes in atherogenesis.
Our scientists have well established flow cytometric techniques to measure platelet-leucocyte interactions in fixed whole blood samples.
The assay can also be performed using a 96-well plate format in combination with whole blood aggregation and, therefore, can offer measurement of platelet-leucocyte conjugates in small volumes of whole blood providing the benefit of multiple sample testing.
Get in touch with our scientific team to understand how our established flow techniques can aid your drug discovery projects.
Afghani M et al (2015)
A new approach to measuring platelet aggregation and platelet-leucocyte conjugate formation in a small volume of fixed whole blood. view paper – page 655
Zhao L et al (2003)
The effects of GPIIb-IIIa antagonists and a combination of three other antiplatelet agents on platelet-leukocyte interactions. Current Medical Research and Opinion, 19(3): 178-186. view paper
Zhao L et al (2001)
Effects of combining three different antiplatelet agents on platelets and leukocytes in whole blood in vitro. British Journal of Pharmacology, 134(2): 353-358. view paper